Sulphated Polysaccharide from Acanthophora spicifera Induced Hevea brasiliensis Defense Responses Against Phytophthora palmivora Infection.
Identifieur interne : 000380 ( Main/Exploration ); précédent : 000379; suivant : 000381Sulphated Polysaccharide from Acanthophora spicifera Induced Hevea brasiliensis Defense Responses Against Phytophthora palmivora Infection.
Auteurs : Sittiporn Pettongkhao [Thaïlande] ; Abdulmuhaimin Bilanglod [Thaïlande] ; Khemmikar Khompatara [Thaïlande] ; Nunta Churngchow [Thaïlande]Source :
- Plants (Basel, Switzerland) [ 2223-7747 ] ; 2019.
Abstract
Elicitors from seaweeds are considered an alternative stimulant of plant defenses against pathogenic infection. Finding new sources of elicitors and exploring their effects on plant defenses is a significant undertaking. In this study, we extracted crude polysaccharide (CPS) from Acanthophora spicifera (a red alga) and tested the effects of the compound on rubber tree (Hevea brasiliensis) defense responses. Accumulations of salicylic acid (SA) and scopoletin (Scp) were measured by HPLC. The expression of SA- and Jasmonic acid (JA)-responsive genes was analyzed by semi-qRT-PCR. Strong anion exchange chromatography and Fourier-transform infrared (FTIR) spectroscopy were used for purification and functional characterization of CPS, respectively. The extracted CPS enhanced rubber tree defenses against Phytophthorapalmivora infection. It induced SA and Scp accumulations and SA-responsive gene expression, but suppressed JA-responsive gene expression. We successfully separated the non-sulphated polysaccharide (F1) from the sulphated polysaccharides (SPS). Both peaks of SPS (F2 and F3) were identified as lambda (λ)-carrageenan. The F3 fraction showed greater elicitor activity on tobacco leaves. It induced SA and Scp accumulations and peroxidase activity but suppressed catalase activity. Furthermore, the purified λ-carrageenan did not cause cell death in tobacco or rubber tree leaves. Therefore, the elicitor from A. spicifera could be an alternative plant stimulant.
DOI: 10.3390/plants8030073
PubMed: 30909469
PubMed Central: PMC6473620
Affiliations:
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Ameen094584@hotmail.com.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla 90112</wicri:regionArea><wicri:noRegion>Songkhla 90112</wicri:noRegion></affiliation></author><author><name sortKey="Khompatara, Khemmikar" sort="Khompatara, Khemmikar" uniqKey="Khompatara K" first="Khemmikar" last="Khompatara">Khemmikar Khompatara</name><affiliation wicri:level="1"><nlm:affiliation>Office of Agricultural Research and Development Region 8, Department of Agriculture, Ministry of Agriculture and Cooperatives, Hat-Yai, Songkhla 90110, Thailand. kjoy2000@hotmail.com.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Office of Agricultural Research and Development Region 8, Department of Agriculture, Ministry of Agriculture and Cooperatives, Hat-Yai, Songkhla 90110</wicri:regionArea><wicri:noRegion>Songkhla 90110</wicri:noRegion></affiliation></author><author><name sortKey="Churngchow, Nunta" sort="Churngchow, Nunta" uniqKey="Churngchow N" first="Nunta" last="Churngchow">Nunta Churngchow</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand. nunta.c@psu.ac.th.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla 90112</wicri:regionArea><wicri:noRegion>Songkhla 90112</wicri:noRegion></affiliation></author></analytic><series><title level="j">Plants (Basel, Switzerland)</title><idno type="ISSN">2223-7747</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Elicitors from seaweeds are considered an alternative stimulant of plant defenses against pathogenic infection. Finding new sources of elicitors and exploring their effects on plant defenses is a significant undertaking. In this study, we extracted crude polysaccharide (CPS) from <i>Acanthophora spicifera</i> (a red alga) and tested the effects of the compound on rubber tree (<i>Hevea brasiliensis</i>) defense responses. Accumulations of salicylic acid (SA) and scopoletin (Scp) were measured by HPLC. The expression of SA- and Jasmonic acid (JA)-responsive genes was analyzed by semi-qRT-PCR. Strong anion exchange chromatography and Fourier-transform infrared (FTIR) spectroscopy were used for purification and functional characterization of CPS, respectively. The extracted CPS enhanced rubber tree defenses against <i>Phytophthora</i><i>palmivora</i> infection. It induced SA and Scp accumulations and SA-responsive gene expression, but suppressed JA-responsive gene expression. We successfully separated the non-sulphated polysaccharide (F1) from the sulphated polysaccharides (SPS). Both peaks of SPS (F2 and F3) were identified as lambda (λ)-carrageenan. The F3 fraction showed greater elicitor activity on tobacco leaves. It induced SA and Scp accumulations and peroxidase activity but suppressed catalase activity. Furthermore, the purified λ-carrageenan did not cause cell death in tobacco or rubber tree leaves. Therefore, the elicitor from <i>A. spicifera</i> could be an alternative plant stimulant.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">30909469</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>29</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Print">2223-7747</ISSN><JournalIssue CitedMedium="Print"><Volume>8</Volume><Issue>3</Issue><PubDate><Year>2019</Year><Month>Mar</Month><Day>22</Day></PubDate></JournalIssue><Title>Plants (Basel, Switzerland)</Title><ISOAbbreviation>Plants (Basel)</ISOAbbreviation></Journal><ArticleTitle>Sulphated Polysaccharide from <i>Acanthophora spicifera</i> Induced <i>Hevea brasiliensis</i> Defense Responses Against <i>Phytophthora palmivora</i> Infection.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E73</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/plants8030073</ELocationID><Abstract><AbstractText>Elicitors from seaweeds are considered an alternative stimulant of plant defenses against pathogenic infection. Finding new sources of elicitors and exploring their effects on plant defenses is a significant undertaking. In this study, we extracted crude polysaccharide (CPS) from <i>Acanthophora spicifera</i> (a red alga) and tested the effects of the compound on rubber tree (<i>Hevea brasiliensis</i>) defense responses. Accumulations of salicylic acid (SA) and scopoletin (Scp) were measured by HPLC. The expression of SA- and Jasmonic acid (JA)-responsive genes was analyzed by semi-qRT-PCR. Strong anion exchange chromatography and Fourier-transform infrared (FTIR) spectroscopy were used for purification and functional characterization of CPS, respectively. The extracted CPS enhanced rubber tree defenses against <i>Phytophthora</i><i>palmivora</i> infection. It induced SA and Scp accumulations and SA-responsive gene expression, but suppressed JA-responsive gene expression. We successfully separated the non-sulphated polysaccharide (F1) from the sulphated polysaccharides (SPS). Both peaks of SPS (F2 and F3) were identified as lambda (λ)-carrageenan. The F3 fraction showed greater elicitor activity on tobacco leaves. It induced SA and Scp accumulations and peroxidase activity but suppressed catalase activity. Furthermore, the purified λ-carrageenan did not cause cell death in tobacco or rubber tree leaves. Therefore, the elicitor from <i>A. spicifera</i> could be an alternative plant stimulant.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Pettongkhao</LastName><ForeName>Sittiporn</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand. sit_ti_pon@windowslive.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bilanglod</LastName><ForeName>Abdulmuhaimin</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand. Ameen094584@hotmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Khompatara</LastName><ForeName>Khemmikar</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Office of Agricultural Research and Development Region 8, Department of Agriculture, Ministry of Agriculture and Cooperatives, Hat-Yai, Songkhla 90110, Thailand. kjoy2000@hotmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Churngchow</LastName><ForeName>Nunta</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand. nunta.c@psu.ac.th.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>-</GrantID><Agency>The Excellent Scholarships in Biochemistry, Department of Biochemistry, Faculty of Science, Prince of Songkla University, Thailand</Agency><Country></Country></Grant><Grant><GrantID>-</GrantID><Agency>The Graduate School of Prince of Songkla University, Thailand</Agency><Country></Country></Grant><Grant><GrantID>PHD/0067/2556</GrantID><Agency>The Royal Golden Jubilee Graduated Program (RGJ-PHD) through the Thailand Research Fund (TRF)</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>03</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Plants (Basel)</MedlineTA><NlmUniqueID>101596181</NlmUniqueID><ISSNLinking>2223-7747</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Acanthophora spicifera</Keyword><Keyword MajorTopicYN="N">Hevea brasiliensis</Keyword><Keyword MajorTopicYN="N">Phytophthora palmivora</Keyword><Keyword MajorTopicYN="N">induced resistance</Keyword><Keyword MajorTopicYN="N">sulphated polysaccharide</Keyword><Keyword MajorTopicYN="N">λ-carrageenan</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>02</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>03</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>03</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>3</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>3</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>3</Month><Day>27</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30909469</ArticleId><ArticleId 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